Role of FTL_0895 in Francisella tularensis Susceptibility to Resazurin
نویسندگان
چکیده
Resistance to antibiotic treatments coupled with the decline in discovery has resulted a steady increase deaths caused by once “curable” bacterial infections. Developing new drugs is crucial prevent more loss of life future. We discovered compound resazurin exhibits antimicrobial activity against gram-negative bacteria including Francisella tularensis (Ft), however, certain strains Ft have developed resistance resazurin. Understanding how Ftdevelops will help defining mechanism which elicits its effect. Whole genome sequencing resazurin-resistant (Rzr) LVS mutants revealed 93% isolates sequenced possessed mutations coding regions FTL_0421, FTL_0895, and FTL_1504. The focus my project was explore role FTL_0895 susceptibility. To confirm this gene plays reduced susceptibility Rzr resazurin, wild-type copy cloned into shuttle vector pABST thenelectroporated one mutants, Rzr1. Complementation did not restore sensitivity Rzr1 strain suggesting that mutation alone responsible for Therefore, we different pMQ2 so can complement back combination either FTL_1504 or FTL_0421. Agar dilution time kill assays be conducted on resulting Rzr-complemented determine their
منابع مشابه
The use of resazurin as a novel antimicrobial agent against Francisella tularensis
The highly infectious and deadly pathogen, Francisella tularensis, is classified by the CDC as a Category A bioterrorism agent. Inhalation of a single bacterium results in an acute pneumonia with a 30-60% mortality rate without treatment. Due to the prevalence of antibiotic resistance, there is a strong need for new types of antibacterial drugs. Resazurin is commonly used to measure bacterial a...
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ژورنال
عنوان ژورنال: Proceedings of the West Virginia Academy of Science
سال: 2023
ISSN: ['0096-4263', '2473-0386']
DOI: https://doi.org/10.55632/pwvas.v95i2.1009